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What is the Olink Flex Customized IFN Stimulation Panel
Customized panel for human
The Olink Flex Customized IFN Stimulation Panel utilizes advanced multiplex proteomics to simultaneously measure 20 biologically relevant biomarkers (including CCL2, IL-15 and IL-6) from minimal sample volumes (1μL plasma/serum), processing 40 samples per run. The platform delivers exceptional sensitivity (<1 pg/mL detection) and precision (<5% CV) using NPX quantification technology. With 208 pre-validated immune markers available for selection, researchers can create tailored 15-21 analyte configurations to investigate interferon-mediated responses. This adaptable system enables detailed exploration of immune modulation mechanisms while ensuring consistent, high-quality data output suitable for various resistance mechanism studies.
Features of the pane
- Species: Designed for human proteome analysis.
- Design: Panel assembly enabled through Olink Insight.
- Proteins: 20 pre-selected proteins associated with immune response modulation.
- Sample: Only 1 µL of plasma or serum per sample.
- Readout: Quantified using NPX (Normalized Protein Expression).
- Platform: Powered by the Olink Signature Q100 platform.
List of 20 Human Derived Biomarkers
Protein category
The Olink Flex Customized IFN Stimulation Panel quantifies 20 strategically selected protein biomarkers organized into key functional categories: Interferon-Stimulated Genes (ISGs), Antiviral Response Mediators, Inflammatory Cytokines/Chemokines, Immune Checkpoint Regulators, Cell Signaling Molecules, Acute Phase Proteins, Growth Factors, and Immune Cell Activation Markers (see Table. List of Olink Flex Customized IFN Stimulation Panel). These targets encompass human proteins involved in fundamental biological mechanisms related to interferon signaling research, covering processes such as viral defense pathways, immune cell communication, inflammatory regulation, and cellular stress responses. The panel incorporates well-characterized biomarkers associated with type I/II/III IFN responses, JAK-STAT signaling cascades, and innate immune activation through rigorous scientific validation and literature review, offering researchers a robust solution for high-throughput proteomic studies in immunology and host-pathogen interaction research.
Table. List of Olink Flex Customized IFN Stimulation Panel
| UniProt ID | Gene | Protein name | Customized Olink Flex panel |
| P05231 | IL6 | Interleukin-6 | IFN stimulation |
| P01137 | TGFb1 | Transforming growth factor beta-1 proprotein | IFN stimulation |
| P24394 | IL4RA | Interleukin-4 receptor subunit alpha | IFN stimulation |
| P13232 | IL7 | Interleukin-7 | IFN stimulation |
| P50591 | TNFSF10 | Tumor necrosis factor ligand superfamily member 10 | IFN stimulation |
| P40933 | IL15 | Interleukin-15 | IFN stimulation |
| P35318 | ADM | Pro-adrenomedullin | IFN stimulation |
| O15455 | TLR3 | Toll-like receptor 3 | IFN stimulation |
| P48023 | FASLG | Tumor necrosis factor ligand superfamily member 6 | IFN stimulation |
| Q9UQV4 | LAMP3 | Lysosome-associated membrane glycoprotein 3 | IFN stimulation |
| P33681 | CD80 | T-lymphocyte activation antigen CD80 | IFN stimulation |
| P12544 | GZMA | Granzyme A | IFN stimulation |
| Q14790 | CASP8 | Caspase-8 | IFN stimulation |
| P13500 | CCL2 | C-C motif chemokine 2 | IFN stimulation |
| P80998 | CCL7 | C-C motif chemokine 7 | IFN stimulation |
| Q99731 | CCL19 | C-C motif chemokine 19 | IFN stimulation |
| Q07325 | CXCL9 | C-X-C motif chemokine 9 | IFN stimulation |
| P02778 | CXCL10 | C-X-C motif chemokine 10 | IFN stimulation |
| O14625 | CXCL11 | C-X-C motif chemokine 11 | IFN stimulation |
| O43927 | CXCL13 | C-X-C motif chemokine 13 | IFN stimulation |
Protein Functions
Biological process
primarily involved in critical biological processes including interferon-mediated immune responses, cellular signaling cascades, chemotaxis and leukocyte recruitment, lymphocyte activation and differentiation, apoptotic regulation, pathogen recognition through pattern recognition receptors, extracellular matrix interactions, and cytokine-cytokine receptor interactions.
Disease area
particularly relevant for scientific investigation across multiple disease research areas, including but not limited to viral pathogenesis studies, autoimmune condition research, inflammatory disorder investigations, immunological surveillance mechanisms, and host-pathogen interaction analyses.
The Application of Olink Flex Customized IFN Stimulation Panel.
The Olink Flex Customized IFN Stimulation Panel analyzes IFN-related serum protein biomarkers, offering researchers valuable insights into:
- Investigation of interferon-mediated immune responses across various viral infection models, enabling researchers to characterize host defense mechanisms and pathogen-host interactions;
- Exploration of autoimmune-associated signaling pathways by profiling key biomarkers involved in immune dysregulation and inflammatory processes;
- Identification of protein signatures related to immune cell activation for studying T-cell polarization, cytokine networks, and chemotaxis in experimental systems.
Workflow of Olink Proteomics
Why Creativ Proteomics
Comprehensive Biomarker Coverage
Includes 20 essential IFN-related proteins, covering key pathways in immune response and viral defense mechanisms.
Rigorous Quality Control
Multi-stage QC protocols ensure data consistency, including inter-plate normalization and sample integrity validation.
Flexible Research Applications
>Supports studies on viral infections, autoimmune mechanisms, and immune cell activation dynamics.
Enhanced Data Interpretation Tools
Includes visualization and analysis resources for streamlined IFN pathway investigation
Demo Results of Olink Data
Figure 1: The Olink immunoassay shows a volcanic plot of differential expression of plasma markers. (Rozeman, E. A., et al. 2021)
Figure 2: Detection of circulating immunogenic cell death-related proteins in SBRT-treated patients with stage I NSCLC. (Vaes, R. D. W., et al. 2021)
Sample Requirements
| Sample Type | Recommended Sample Size | Sample Quality | Pre-treatment and Storage | Sample Transport |
| Plasma/Serum/Body Fluid | Minimum 40 µL sample volume required | Optimal protein range: 0.5–1 mg/mL | Aliquot into sterile vials; store at -80°C | Dry ice shipment (-80°C) for foil-sealed samples |
| Tissue | ||||
| Cells | ||||
| Exosomes | ||||
| Other |
FAQs
What are the components of the Olink Flex IFN Stimulation Panel controls, and how are they used in data analysis?
The panel includes triplicate pooled human plasma controls supplemented with recombinant proteins for key IFN-related biomarkers. These controls are run on each plate to monitor inter-plate variation, validate assay precision (CV <5%), and ensure consistent NPX (Normalized Protein Expression) quantification across batches. Detailed validation data are available on the Olink platform.
How were the biomarkers in the IFN Stimulation Panel selected?
Biomarkers were curated through:
- Public databases (UniProt, Gene Ontology) for IFN pathway relevance.
- Expert input on immune response mechanisms (e.g., JAK-STAT signaling, antiviral defense).
- Rigorous antibody validation for specificity, sensitivity, and reproducibility.
The final panel combines well-characterized and exploratory targets to balance robustness and discovery potential.
What research applications does this panel support?
- Viral infection models (host-pathogen interactions, IFN-mediated defense).
- Autoimmune research (dysregulated IFN signaling in experimental systems).
- Immuno-oncology studies (T-cell activation, cytokine networks).
- Innate immunity investigations (e.g., Toll-like receptor pathways).
- Therapeutic mechanism research (e.g., cytokine modulation in vitro).
How customizable is the panel for specific study needs?
Researchers can select 15–21 analytes from 208 pre-validated immune markers, tailoring the panel to focus on specific IFN subtypes (I/II/III), chemokine networks, or immune checkpoint proteins. Fixed + customizable slots ensure flexibility without compromising data qual.
References
- Rozeman, E. A., Hoefsmit, E. P., Reijers, I. L. M., et al. (2021). Survival and biomarker analyses from the OpACIN-neo and OpACIN neoadjuvant immunotherapy trials in stage III melanoma. Nature medicine, 27(2), 256–263. https://doi.org/10.1038/s41591-020-01211-7
- Vaes, R. D. W., Reynders, K., Sprooten, J., et al. (2021). Identification of Potential Prognostic and Predictive Immunological Biomarkers in Patients with Stage I and Stage III Non-Small Cell Lung Cancer (NSCLC): A Prospective Exploratory Study. Cancers, 13(24), 6259. https://doi.org/10.3390/cancers13246259