Olink Target 96 – Comprehensive Protein Profiling Across 15 Panels

Olink Target 96 Panels – High-Throughput Protein Profiling for Research

Olink Target 96 panels deliver high-multiplex protein analysis, enabling researchers to measure 92 biomarkers per panel using as little as 1–3 μL of sample. Whether you're exploring immune response, oncology, neurology, cardiometabolic health, or organ injury, Olink Target 96 provides a cost-efficient, sensitive, and scalable solution.

92 proteins per panel – broad pathway coverage
15 themed panels for human and mouse research
Ultra-sensitive PEA technology for reliable results

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Olink Target 96 Panels platform at Creative Proteomics

Overview
Panels
Why Choose
Sample Requirements
Demo Data
FAQ

What is Olink Target 96?

Olink Target 96 is a protein biomarker discovery platform powered by Proximity Extension Assay (PEA) technology, which pairs specificity with ultra-sensitivity. Each panel covers 92 carefully curated proteins, giving researchers a high-resolution view of critical biological pathways.

Low sample requirement: only 1–3 μL of plasma, serum, CSF, or other sample types
Flexible applications: from biomarker discovery to drug development
Validated across human and mouse models for translational research

Unlike traditional immunoassays, Olink's dual antibody binding and DNA tagging system minimizes off-target effects, delivering data you can trust.

Explore All Olink Target 96 Panels

15 focused panels – one powerful platform.

Researchers can choose from panels that address major areas of biomedical research. Each panel includes 92 validated proteins, allowing you to profile disease pathways, validate biomarkers, or assess drug response in a single experiment.

Immune & Inflammation

Olink Target 96 Immune Response Panel - profiles immune activation pathways
Olink Target 96 Inflammatory Panel - targets cytokines and inflammation mediators
Olink Target 96 Immuno-Oncology Panel - links immune function to tumor biology

Oncology & Cell Biology

Olink Target 96 Oncology II Panel - core biomarkers for solid tumor studies
Olink Target 96 Oncology III Panel - expanded oncology protein coverage
Olink Target 96 Cell Regulation Panel - markers involved in cell cycle and apoptosis

Cardiovascular & Metabolism

Olink Target 96 Cardiometabolic Panel – connects metabolic and cardiovascular health
Olink Target 96 Cardiovascular II Panel – proteins relevant to heart disease research
Olink Target 96 Cardiovascular III Panel – advanced cardiac biomarker insights
Olink Target 96 Metabolism Panel – targets metabolic signaling pathways

Neurology & Exploratory

Olink Target 96 Neurology Panel – biomarkers for neurodegeneration and CNS studies
Olink Target 96 Neuro Exploratory Panel – discovery panel for novel neuro proteins
Olink Target 96 Organ Damage Panel – proteins indicating tissue and organ injury
Olink Target 96 Development Panel – exploratory panel for emerging research needs
Olink Target 96 Mouse Exploratory Panel – preclinical mouse-specific protein panel

Workflow of Olink Proteomics

Olink Proteomics workflow with six steps from sample collection to report delivery

Why Choose Olink Target 96?

When choosing a proteomics solution, you need accuracy, efficiency, and scalability. Olink Target 96 offers:

Minimal sample volume

only 1–3 μL required

Cost-efficient multiplexing

analyze 92 proteins in a single run

Ultra-sensitive detection

PEA technology reduces off-target effects

Broad applicability

validated for both human and mouse research

Whether you're validating oncology biomarkers, tracking inflammatory changes, or mapping neurological pathways, Olink Target 96 delivers robust, reproducible results.

Sample Requirements for Olink Target 96 Panels

Sample Type	Volume per Panel	Matrix / Notes	Storage Conditions
Serum	Minimum 50 µL	Use clot activator tubes; avoid haemolysis	Store at −80 °C
Plasma	Minimum 50 µL	EDTA-plasma or heparin plasma preferred	Store at −80 °C
CSF (Cerebrospinal Fluid)	Minimum 50 µL	Collect under sterile conditions	Store at −80 °C
Cell Culture Supernatant	Minimum 50–100 µL	Clarify by centrifugation to remove debris	Store at −20 °C to −80 °C
Urine (optional)	Minimum 100 µL	Collect midstream sample, centrifuge before use	Store at −80 °C
Other Matrices	Case-by-case	Contact us for guidance (e.g. saliva, synovial fluid, tissue homogenates)	Store at −80 °C

Sample Handling Guidelines

Avoid freeze–thaw cycles: More than two freeze–thaw events can affect protein stability.
Labeling: Each tube must be clearly labeled with sample ID, matrix type, and collection date.
Shipping: Send samples on dry ice to maintain cold chain integrity.
Volume buffer: Provide an additional 10–15 µL to allow for QC and repeat analysis if needed.

Experience Real Olink Target 96 Data

To help you evaluate the platform's performance, we present actual demo results from an Olink Target 96 run:

Volcano plots: highlight significantly changed proteins post-treatment vs. control (Panel B).
Heatmaps: display protein expression across sample groups, clustered by expression pattern.
Scatter charts: show reproducibility of assay duplicates for critical biomarker proteins (Panel A).

These visualizations reflect:

Robust NPX-based relative quantification
Minimal technical variation across replicates
Clear protein expression trends across experimental groups

How to Explore the Demo Visuals

Data Visualization	What It Shows	Research Value
Volcano Plot	Up/down-regulated proteins vs. statistical significance	Identify target hits for follow-up
Heatmap	Expression clusters across samples	Explore group-specific protein signatures
Scatter Plot	Correlation between replicate measurements	Confirms data reliability and QC consistency

Note: Arrays include internal and external controls for run quality.

FAQ – Everything You Should Know About Olink Target 96

Q1: What sample types are compatible with Olink Target 96?

Olink Target 96 is fully validated for use with EDTA-plasma and serum, but has also been successfully applied to cerebrospinal fluid, urine, cell lysates, synovial fluid, tissue homogenates, exosomes, microdialysis fluid, saliva, and more.

Q2: How many samples can be analyzed per Target 96 kit?

Each kit supports up to 88 samples, plus 6 built-in controls (3 negative and 3 plate controls). To monitor technical consistency, we typically recommend running 1–2 external QC samples per plate—this may reduce the number of test samples slightly.

Q3: Is the data quantification absolute or relative? What is NPX?

Olink Target 96 provides relative quantification using NPX (Normalized Protein eXpression) values on a log₂ scale. NPX reflects relative protein abundance between samples—not absolute concentration. To convert to linear scale, use the formula: 2^NPX.

Q4: What controls and QC measures are built into Olink Target 96?

Each plate includes internal controls for quality:

Incubation Controls monitor antibody binding steps
Extension & Amplification Controls help normalize across plates
A sample fails QC if control deviations exceed ± 0.3 NPX. If more than 1/6 samples per plate are flagged, the entire run is flagged for review or rerun.

Q5: What assay sensitivity and dynamic range can I expect?

Olink's PEA technology achieves detection in the fg/mL range, over a dynamic range spanning up to 10 logs. Its sensitivity often matches or surpasses traditional ELISA assays, even for low-abundance biomarkers.

Q6: How reproducible are the measurements?

Most assays on Olink Target 96 panels show intra- and inter-plate CVs below 10%, with intraclass correlation coefficients (ICCs) above 0.8, meaning single-run data are reliable enough that technical replicates are generally unnecessary.

Q7: Can data from different study batches or panels be compared across runs?

Yes. Olink recommends sample randomisation and normalization strategies:
Use intensity normalization for randomized studies
Include 8–16 bridging samples when comparing non-randomised or multi-batch studies
This helps reduce batch effects and support robust longitudinal or cross-panel analyses.

Q8: What are on‑target and off‑target effects, and how does Olink reduce them?

"On‑target effects" are accurate protein detections; "off‑target effects" are non-specific signals. Olink's dual-antibody PEA system with DNA barcoding ensures both antibodies must bind a protein before amplification, greatly minimizing false positives and non-specific binding—yielding trustworthy results every time.

Q9: Does Olink offer panels for research in mouse or rat models?

Yes—Target 96 Mouse Exploratory Panel is suitable for mice and has also been shown to work with rat samples. Validation and calibration data support cross-species usage, enabling preclinical and translational studies.

Q10: Can I use official human panels for non-human species like mouse?

Human panels are not officially validated for non-human samples, though some cross-reactivity may occur. Olink recommends using species-specific panels (e.g. Mouse Exploratory) for rigorous preclinical accuracy. Use of human panels with animal samples is permitted only at the user's discretion.

Q11: What is the turnaround time from sample receipt to data delivery?

Once we receive your samples (recommended shipping volume: 40–80 µL per sample), our team will begin quality control, data processing in NPX Manager, and result preparation immediately. The final timeline will depend on the panel selection, study size, and sample type, and will be confirmed during project planning to meet your research schedule.

Q12: How much does Olink Target 96 cost?

Pricing varies based on panel selection, number of samples, and study requirements. Partial plate runs are available if you have fewer than 88 samples. Please contact us for a custom quote tailored to your project needs.

* For research purposes only, not intended for clinical diagnosis, treatment, or individual health assessments.

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