Olink Target 96 Cardiovascular III Panel

What is the Olink Target 96 Cardiovascular III Panel

Customized panel for human

The Olink Target 96 Cardiovascular III Panel is designed to accurately quantify proteins, with comprehensive biomarker details accessible on our company website. Employing advanced Biomarker technology, the panel analyzes 92 proteins through a three-step process: incubation, extension/amplification, and detection. During the incubation phase, DNA-labeled antibody pairs are added to the sample and incubated overnight to bind specific target proteins. The next day, extension and amplification steps produce unique DNA reporter sequences for each protein, followed by preamplification using standard PCR. Detection is performed using high-throughput real-time qPCR on the Olink Signature Q100 System to measure the DNA reporter sequences. To ensure unbiased analysis, samples were randomly allocated across plates. Data quality control and normalization were conducted using internal extension and interplate controls, addressing both intra- and inter-batch variability. Protein levels are expressed as normalized protein expression (NPX) values, calculated on a log2 scale for accurate and reliable interpretation.

 Features of the pane

• Species: Primarily tested for human proteins; cross-reactivity with other species is not confirmed.  
• Proteins: Measures 92 protein biomarkers simultaneously in a single run.
• Sample: Just 1µL of plasma, serum, or comparable biofluids is required.
• Readout: Delivers data in normalized protein expression (NPX) units, ensuring reliable quantification of protein abundance.
• Platform: Optimized for use with the Olink Signature Q100 platform for streamlined performance.

List of 92 human derived biomarkers

Protein category

The Olink Target 96 Oncology III Panel includes 92 proteins categorized into nine main groups:

the Cytokines & Growth Factors (7), Receptors (18), the enzymes (17), Extracellular Matrix Proteins (6), Immune-related Proteins (10), Transport & Binding Proteins (6), Enzyme Inhibitors (5), Signaling Molecules (9), and other functional proteins (14). These protein biomarkers were selected by taking into account both their dynamic range in the sample and their closeness to cardiovascular disease. The Cardiovascular Disease-III panel contains known human cardiovascular and inflammatory markers as well as candidate proteins with great potential as cardiovascular disease markers. Each protein was carefully selected by experts in the field. Each of the low-abundance protein analytes of interest has been evaluated in terms of sample material, specificity, precision, sensitivity, dynamic range, matrix effects, and interference.

Table. List of Olink Target 96 Cardiovascular III Panel.

Protein Functions

Biological process

Primarily associated with immune systerm diseases, signal transduction, innate immune system, and cytokine signaling in immune systerm.

Disease area

Primarily associated with carsiovascule, metsbolic, cancer, imuune, and rheumatoid arthritis.

Workflow of Olink Proteomics

Demo Results of Olink Data

Diabetic AVs show sex-specific molecular pathway differences. (Miriam Goñi-Olóriz, et al. 2025)

Case Study

Cardiomyocyte and stromal cell cross-talk influences the pathogenesis of arrhythmogenic cardiomyopathy: a multi-level analysis uncovers DLK1-NOTCH pathway role in fibro-adipose remodelling

Journal: Cell death discovery
Year: 2024

• Background
• Results

ACM is a life-threatening, genetically determined disease primarily caused by gene mutations in desmosomal genes such as PKP2. Due to the complex etiology of ACM, the pathogenesis has not been fully elucidated, and there are currently no treatment methods targeting its causes. Various types of cardiomyocytes affected by gene mutations, such as cardiomyocytes (CM) and cardiac mesenchymal stem cells (cMSC), each contribute to the ACM phenotype, driving functional abnormalities and fibrofatty replacement, respectively. However, the relative importance of changes in CM and cMSC, as well as their interactions in disease progression, remain poorly understood.

Cell-to-cell communication occurs through paracrine signaling, involving soluble mediators. To investigate the efficient release of these mediators, we conducted a secretome analysis of co-cultures using Olink technology. Conditioned media were collected, and 92 cardiovascular-related factors were screened, with 36 showing differential secretion across four co-culture groups. Figure 5A illustrates the secretion diversity, while levels of the 36 factors are detailed. We focused on factors linked to ACM-dependent phenotypic changes. Col1a1, MMP9, and TIMP4 secretion increased in ACM-containing cMSC co-cultures (Figures 5B-D). Fibrosis- and mechanosensory-related factors, such as uPAR and PECAM-1, were elevated in ACM cMSCs and HC cMSCs, respectively (Figures 5E-F). GDF-15 and LDL receptors were upregulated in ACM cMSC co-cultures, while MBs were more abundant in ACM CM co-cultures (Figures 5G-I). Notably, DLK1 was uniquely secreted by HC CM co-cultures (Figure 5L).

Figure 1. Proteins involved in co-culturing paracrine interactions. (Angela Serena Maione, et al. 2024)

FAQs

Why Creative Proteomics

Cutting-Edge Data Integration

Combining Olink proteomics with multi-omics data enables holistic disease insights and accelerates therapeutic target discovery.

Customized Analytical Pipelines

Custom bioinformatics pipelines are created to enable precise data processing and generate actionable insights in complex research projects.

Collaborative Research Partnerships

collaborates with academia and industry, offering co-development and innovative solutions to achieve impactful research outcomes.

Sample Requirements

References

1. Maione, A. S., Iengo, L., Sala, L., et al. (2024). Cardiomyocyte and stromal cell cross-talk influences the pathogenesis of arrhythmogenic cardiomyopathy: a multi-level analysis uncovers DLK1-NOTCH pathway role in fibro-adipose remodelling. Cell death discovery, 10(1), 484. https://doi.org/10.1038/s41420-024-02232-8 
2. Goñi-Olóriz, M., Garaikoetxea Zubillaga, M., San Ildefonso-García, S., et al. (2025). Chemerin is a new sex-specific target in aortic stenosis concomitant with diabetes regulated by the aldosterone/mineralocorticoid receptor axis. American journal of physiology. Heart and circulatory physiology, 328(3), H639–H647. https://doi.org/10.1152/ajpheart.00763.2024