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What is the Olink Flex Customized Th1/Th2/Th17 Panel
Customized panel for human
The Olink Flex Customized Th1/Th2/Th17 Panel employs multiplex proteomics to quantify 21 key biomarkers characterizing T-helper cell populations, including core cytokines and novel targets, using just 1μL plasma/serum with 40-sample throughput. This high-sensitivity platform achieves <1 pg/mL detection limits and <5% CV reproducibility through normalized protein expression (NPX). Researchers can customize panels by selecting 15-21 analytes from 208 validated immune markers, enabling tailored investigations of Th1/Th2/Th17 biology. The flexible design facilitates focused studies of immune polarization mechanisms in various research contexts while maintaining Olink's gold-standard data quality. This solution supports comprehensive analysis of T-cell mediated immune responses across health and disease research applications.
Features of the pane
- Species: Optimized for human studies.
- Design: Panel configuration via Olink Insight;
- Proteins: 21 biomarkers characterizing T-helper cell populations.
- Sample: 1 µL plasma/serum required.
- Readout: NPX-normalized quantification.
- Platform: Olink Signature Q100 platform exclusive.
List of 21 Human Derived Biomarkers
Protein category
The Olink Flex Customized Th1/Th2/Th17 Panel measures 21 strategically selected protein biomarkers organized into key functional categories: Th1-Associated Cytokines (including IFN-γ and IL-2), Th2-Associated Markers (including IL-4, IL-5, and IL-13), Th17-Related Proteins (including IL-17A and IL-17F), Chemokine Ligands/Receptors, Pleiotropic Cytokines, and Immune Checkpoint Regulators (see Table. List of Olink Flex Customized Th1/Th2/Th17 Panel). These targets encompass human proteins involved in fundamental immunological mechanisms of T-helper cell differentiation and function, covering processes such as lymphocyte activation, cytokine signaling cascades, immune cell recruitment, and inflammatory response modulation. The panel incorporates rigorously validated biomarkers associated with Th1/Th2/Th17 polarization, including transcription factors, surface markers, and soluble mediators through systematic literature review and proteomic verification, offering researchers a comprehensive solution for investigating immune cell dynamics and cytokine networks in various experimental systems and disease models. This carefully curated selection enables multidimensional profiling of T-cell mediated immunity while maintaining flexibility for customized panel configurations tailored to specific research objectives in immunology and autoimmune studies.
Table. List of Olink Flex Customized Th1/Th2/Th17 Panel
| UniProt ID | Gene | Protein name | Customized Olink Flex panel |
| Q96PD4 | IL17F | Interleukin-17F | Th1/Th2/Th17 |
| O95760 | IL33 | Interleukin-33 | Th1/Th2/Th17 |
| Q13241 | KLRD1 | Natural killer cells antigen CD94 | Th1/Th2/Th17 |
| Q8TD46 | CD200R1 | Cell surface glycoprotein CD200 receptor 1 | Th1/Th2/Th17 |
| P32970 | CD70 | CD70 antigen | Th1/Th2/Th17 |
| P01579 | IFNG | Interferon gamma | Th1/Th2/Th17 |
| P01375 | TNF | Tumor necrosis factor | Th1/Th2/Th17 |
| P04141 | CSF2 | Granulocyte-macrophage colony-stimulating factor | Th1/Th2/Th17 |
| P60568 | IL2 | Interleukin-2 | Th1/Th2/Th17 |
| P29459_P29460 | IL12A_IL12B | Interleukin-12 | Th1/Th2/Th17 |
| P13500 | CCL2 | C-C motif chemokine 2 | Th1/Th2/Th17 |
| P10147 | CCL3 | C-C motif chemokine 3 | Th1/Th2/Th17 |
| P01374 | LTA | Lymphotoxin-alpha | Th1/Th2/Th17 |
| P51671 | CCL11 | Eotaxin | Th1/Th2/Th17 |
| P05231 | IL6 | Interleukin-6 | Th1/Th2/Th17 |
| P05112 | IL4 | Interleukin-4 | Th1/Th2/Th17 |
| P05113 | IL5 | Interleukin-5 | Th1/Th2/Th17 |
| P22301 | IL10 | Interleukin-10 | Th1/Th2/Th17 |
| P35225 | IL13 | Interleukin-13 | Th1/Th2/Th17 |
| Q9H293 | IL25 | Interleukin-25 | Th1/Th2/Th17 |
| Q16552 | IL17A | Interleukin-17A | Th1/Th2/Th17 |
Protein Functions
Biological process
primarily involved in fundamental immunological processes including T-helper cell differentiation, innate immune cell activation, cytokine-mediated signaling pathways, chemotaxis and leukocyte recruitment, immune regulation and tolerance, and hematopoietic cell proliferation/differentiation
Disease area
particularly relevant for scientific investigations across multiple research domains including autoimmune mechanisms, allergic inflammation, infectious disease responses, hematopoietic disorders, chronic inflammatory conditions, and immune regulation studies
The Application of Olink Flex Customized Th1/Th2/Th17 Panel.
The Olink Flex Customized Th1/Th2/Th17 Panel enables precise quantification of T-helper cell-associated biomarkers, providing researchers with a powerful tool for:
- Identification of cytokine signatures linked to Th1/Th2/Th17 immune polarization;
- Mechanistic investigation of immune dysregulation in autoimmune and inflammatory responses;
- Discovery of molecular networks underlying T-cell-mediated immunity;
- Stratification of immune phenotypes based on cytokine profiles.
Workflow of Olink Proteomics
Why Creativ Proteomics
Multi-Stage Quality Control
Implements 12-point validation protocols with plate-to-plate normalization, maintaining <5% CV for 98% of T-helper cell cytokines.
Immune-Specific Analytical Frameworks
Preconfigured workflows for Th1/Th2/Th17 polarization studies, including validated biomarker ratios for immune dysregulation research.
Standardized Immune Metrics
Includes 21 published immune scoring algorithms for Th subset activity quantification.
Integrated Data Solutions
Specialized visualization tools for: Cytokine network mapping, Longitudinal immune response tracking and Cross-cohort comparisons.
Demo Results of Olink Data
Figure 1: To compare the differentially expressed immune-related proteins in the blood of patients with atopic dermatitis in different age groups. (Del Duca, E., et al. 2023)
Figure 2: Age-dependent atherosclerosis-related protein changes in atopic dermatitis. (Del Duca, E., et al. 2023)
Sample Requirements
| Sample Type | Recommended Sample Size | Sample Quality | Pre-treatment and Storage | Sample Transport |
| Plasma/Serum/Body Fluid | Sample volume: 40 µL minimum | Target: 0.5–1 mg/mL protein | Distribute into sterile vials; freeze at -80°C | Dry ice shipment (-80°C) for foil-sealed samples |
| Tissue | ||||
| Cells | ||||
| Exosomes | ||||
| Other |
FAQs
What are the components of the Olink Flex Sample controls and how are they used in data analysis?
A sample control is a pooled human plasma sample with recombinant antigens for some proteins to ensure that all proteins are detected. Sample controls were included in triplicate on each plate. Sample controls were used to assess potential between-batch and between-plate variation, as well as quality control for pg/mL data quantification. Validation data for all panels are available on the Olink website.
How are the biomarkers in the Olink Flex panel selected?
In constructing the new panel, we collected information from widely used public bioinformatics databases such as Uniprot, Human Protein Atlas, Gene Ontology, and DisGeNET.
We selected and evaluated a combination of known and exploratory biomarkers for a suitable antibody that would then need to pass our rigorous validation tests and requirements. The selection process and panel composition were developed with input from experts in each field.
What research applications does it support?
- Autoimmune disease mechanisms (e.g., RA, MS).
- Infectious disease immune responses.
- Allergy and asthma research.
- Cancer immunology (T-cell modulation).
- Therapeutic development (e.g., biologics, checkpoint inhibitors).
Reference
- Del Duca, E., Renert-Yuval, Y., Pavel, A. B., et al. (2023). Proteomic characterization of atopic dermatitis blood from infancy to adulthood. Journal of the American Academy of Dermatology, 88(5), 1083–1093. https://doi.org/10.1016/j.jaad.2022.12.050