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What is the Olink Flex Customized Cytokine Storm Panel
Customized panel for human
The Olink Flex Customized Cytokine Storm Panel utilizes multiplex proteomics technology to quantify 21 key biomarkers that are frequently monitored and found to be elevated in this pathological state, including core cytokines and novel targets, using only 1 μL of plasma/serum, with a throughput of 40 samples. This highly sensitive platform achieves a detection limit of <1 pg/mL and a CV repeatability of <5% with standardized protein expression (NPX). Researchers can select 15~21 analytes from 208 validated immunomarkers to customize the assay panel, enabling individualized studies of cytokine storm biology. The flexible design facilitates focused research on immune polarization mechanisms in a variety of research contexts while maintaining Olink's gold-standard data quality. The solution enables comprehensive analysis of cytokine storms in health and disease research applications.
Features of the pane
- Species: Human proteome-focused assay optimization.
- Design: Custom biomarker selection through Olink Insight interfacet.
- Proteins: 21-plex cytokine and immune response protein panel.
- Sample: Ultra-low volume requirement (1 µL biofluid).
- Readout: Normalized Protein Expression (NPX) quantification.
- Platform: Proprietary PEA technology on Signature Q100.
List of 21 Human Derived Biomarkers
Protein category
The Olink Flex Customized Cytokine Storm Panel measures a comprehensive selection of protein biomarkers organized into distinct functional categories: Pro-inflammatory Cytokines, Anti-inflammatory Regulators, Interferons and Signaling Molecules, Chemotactic Factors, Cytotoxic Mediators, and Immune Cell Activators (see Table. List of Olink Flex Customized Cytokine Storm Panel). These targets encompass human proteins involved in critical immunological processes relevant to inflammation research, covering mechanisms such as cytokine signaling cascades, leukocyte recruitment, immune cell activation and differentiation, and the balance between pro- and anti-inflammatory responses. The panel incorporates well-characterized biomarkers associated with cytokine storm syndromes, hyperinflammatory states, and immune dysregulation through rigorous scientific validation, providing researchers with a robust tool for high-throughput proteomic investigations in fundamental immunology and inflammatory disease research.
Table. List of Olink Flex Customized Cytokine Storm Panel
| UniProt ID | Gene | Protein name | Customized Olink Flex panel |
| P01563 | IFNA2 | Interferon alpha-2 | Cytokine storm |
| P04141 | GM-CSF | Granulocyte-macrophage colony-stimulating factor | Cytokine storm |
| P12544 | GZMA | Granzyme A | Cytokine storm |
| P18510 | IL1-RA | Interleukin-1 receptor antagonist protein | Cytokine storm |
| P22301 | I L-10 | Interleukin-10 | Cytokine storm |
| P05112 | I L-4 | Interleukin-4 | Cytokine storm |
| P01579 | IFNG | Interferon gamma | Cytokine storm |
| P01375 | TNFa | Tumor necrosis factor | Cytokine storm |
| P05231 | IL - 6 | Interleukin-6 | Cytokine storm |
| Q14116 | IL-18 | Interleukin-18 | Cytokine storm |
| P29459_P29460 | IL-12p70 | Interleukin 12,IL-12/P70 | Cytokine storm |
| P60568 | I L-2 | Interleukin-2 | Cytokine storm |
| P01584 | IL-1B | Interleukin-1 beta | Cytokine storm |
| P01583 | IL-1A | Interleukin-1 alpha | Cytokine storm |
| P02778 | CXCL10 | C-X-C motif chemokine 10 | Cytokine storm |
| Q07325 | CXCL9 | C-X-C motif chemokine 9 | Cytokine storm |
| Q14116 | IL-8 | Interleukin-18 | Cytokine storm |
| P10147 | CCL3 | C-C motif chemokine 3 | Cytokine storm |
| P13500 | CCL2 | C-C motif chemokine 2 | Cytokine storm |
| P13236 | CCL4 | C-C motif chemokine 4 | Cytokine storm |
| P01574 | IFNB1 | Interferon beta | Cytokine storm |
Protein Functions
Biological process
primarily involved in key biological processes including innate and adaptive immune responses, inflammatory signaling cascades, leukocyte recruitment and activation, cytokine-mediated cell communication, and immune cell differentiation and regulation.
Disease area
primarily associated with research areas involving dysregulated immune responses, including but not limited to cytokine release syndromes, autoimmune disorders, infectious disease pathogenesis, chronic inflammatory conditions, and hyperinflammatory states observed in experimental models.
The Application of Olink Flex Customized Cytokine Storm Panel.
The Olink Flex Customized Cytokine Storm Panel enables high-plex profiling of inflammatory biomarkers, providing researchers with a powerful tool for:
- Uncovering dynamic cytokine networks in systemic inflammatory responses, aiding mechanistic studies of immune dysregulation;
- Discovery of novel biomarker signatures linked to hyperinflammatory states, supporting translational research in infectious and autoimmune diseases;
- Stratification of molecular phenotypes in experimental models, facilitating precision investigation of cytokine storm drivers and modulators.
Workflow of Olink Proteomics
Why Creativ Proteomics
Disease-Specific Analytical Frameworks
PreconfiguredPreconfigured workflows for autoimmune diseases (RA, IBD), infectious diseases (sepsis, COVID-19), and chronic inflammation streamline study design.
Customizable Configuration
Flexible 15-21 analyte selection from pre-validated markers to tailor panels to specific research needs.
Sample Integrity Validation
Robust protocols ensure sample quality, reducing variability and increasing reproducibility in cytokine storm research.
Researcher Support Tools
Included data interpretation guides and visualization templates for cytokine network analysis.
Demo Results of Olink Data
Figure 1: Integrated plasma and single-cell proteomic analysis across COVID-19 severity levels (mild/moderate/severe). (Feyaerts, D , et al.2022)
Figure 2: Plasma cytokine storm protein levels across study groups. (Byeon, S. K , et al.2022)
Sample Requirements
| Sample Type | Recommended Sample Size | Sample Quality | Pre-treatment and Storage | Sample Transport |
| Plasma/Serum/Body Fluid | Sample requirement: 40 µL | Optimal protein range: 0.5–1 mg/mL | Aliquot into sterile vials and store at -80°C | Dry ice shipment at -80°C for sealed samples |
| Tissue | ||||
| Cells | ||||
| Exosomes | ||||
| Other |
FAQs
What is the composition of the cytokine storm panel controls?
The panel utilizes triplicate pooled human plasma controls with recombinant spike-ins for 12 critical cytokines, ensuring <8% inter-plate CV and maintaining NPX consistency across batches.
What research applications does this panel support?
- Viral infection models (e.g., SARS-CoV-2, influenza cytokine release)
- CRS mechanism studies in immunotherapy research
- Inflammatory cascade analysis in preclinical models
What customization options are available for researchers?
The flexible panel design allows selection of 15-21 analytes from a validated menu of 92 markers, with options to include normalization controls, spike-in references, and three different panel sizes, enabling tailored configurations for specific experimental needs while maintaining the platform's rigorous quality standards
How does the technology handle low-abundance inflammatory markers?
Through proprietary PEA technology, the panel achieves exceptional sensitivity with detection of IL-6 at 0.3 pg/mL and reliable quantification (<15% CV) of 95% of targets below 10 pg/mL, enabling robust measurement of even trace-level cytokines in complex biological samples.
References
- Feyaerts, D., Hédou, J., Gillard, J., et al. (2022). Integrated plasma proteomic and single-cell immune signaling network signatures demarcate mild, moderate, and severe COVID-19. Cell reports. Medicine, 3(7), 100680. https://doi.org/10.1016/j.xcrm.2022.100680
- Byeon, S. K., Madugundu, A. K., Garapati, K., et al. (2022). Development of a multiomics model for identification of predictive biomarkers for COVID-19 severity: a retrospective cohort study. The Lancet. Digital health, 4(9), e632–e645. https://doi.org/10.1016/S2589-7500(22)00112-1