Olink Flex Customized Inflammation in Aging Panel

The Olink Flex Customized inflammatory in Aging panel includes 21 protein biomarkers associated with age-related immune system changes, including CDCP1, STAMBP, RANK, and PLAUR. These biomarkers may provide deeper insights into age-related inflammation and explore molecular mechanisms underlying age-associated biological processes.

As a pioneer in next-generation proteomics, we are proud to introduce the Olink Flex Customized Inflammation in Aging Panel, an advanced research solution enabling scientists to investigate inflammaging mechanisms with unprecedented precision.

This fully customizable panel allows researchers to select from an extensive menu of rigorously validated protein biomarkers involved in age-related inflammatory processes, including senescence-associated secretory phenotype (SASP) factors, chronic low-grade inflammation markers, and immune cell activation proteins. Each analyte has been meticulously evaluated for performance characteristics such as sensitivity (detection limits <1 pg/mL), specificity, dynamic range, and matrix compatibility across various biological samples. The panel's flexible design supports targeted investigations into inflammatory pathways underlying cellular aging, tissue degeneration, and longevity research, offering researchers the freedom to tailor their protein biomarker selection while maintaining Olink's gold-standard data quality for comprehensive exploration of inflammaging biology.

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Panel Features
Panels List
Workflow
Why Creative Proteomics
Demo
Sample Requirements
FAQ

What is the Olink Flex Customized Inflammation in Aging Panel

Customized panel for human

The Olink Flex Customized Inflammation in Aging Panel utilizes advanced multiplex technology to quantify 21 biomarkers associated with immune senescence, including both established and exploratory targets, from just 1μL of plasma/serum (40 samples/run). The platform demonstrates exceptional sensitivity (<1 pg/mL detection) and reproducibility (<5% CV) through normalized protein expression analysis. Researchers can customize panels by selecting 15-21 proteins from 208 validated inflammatory markers, enabling tailored investigations of age-related immune dysfunction. This flexible solution supports comprehensive analysis of inflammaging mechanisms and their potential connections to various aging-associated conditions while maintaining Olink's high-quality standards.

Features of the pane

Species: Human-specific research applications.
Design: Custom configuration via Olink Insight.
Proteins: the top 21 biomarkers relevant to immune senescence.
Sample: 1 µL plasma/serum sample.
Readout: NPX-normalized protein data.
Platform: Olink Signature Q100 platform.

List of 21 Human Derived Biomarkers

Protein category

The Olink Flex Customized Inflammation in Aging Panel quantifies 21 strategically selected protein biomarkers organized into key functional categories: Senescence-Associated Secretory Phenotype (SASP) Factors, Pro-inflammatory Cytokines/Chemokines, Anti-inflammatory Regulators, Acute-Phase Proteins, Complement System Components, Growth Factors, Adhesion Molecules, and Stress Response Proteins (see Table. List of Olink Flex Customized Inflammation in Aging Panel).

These targets encompass human proteins involved in fundamental biological mechanisms of inflammaging, covering processes such as cellular senescence, chronic low-grade inflammation, immune cell dysregulation, tissue remodeling, and oxidative stress responses. The panel incorporates rigorously validated biomarkers associated with age-related inflammatory pathways, including NF-κB signaling, inflammasome activation, mitochondrial dysfunction, and extracellular matrix alterations through systematic literature review and proteomic verification, offering researchers a comprehensive solution for investigating the molecular interplay between aging and inflammatory processes in various biological systems and model organisms. This carefully curated selection enables multidimensional profiling of inflammaging networks while maintaining flexibility for customized panel configurations tailored to specific research objectives in aging biology and longevity studies.

Table. List of Olink Flex Customized Inflammation in Aging Panel

UniProt ID	Gene	Protein name	Customized Olink Flex panel
P01133	EGF	Pro-epidermal growth factor	Inflammation in Aging
P15692	VEGFA	Vascular endothelial growth factor A	Inflammation in Aging
P01730	CD4	T-cell surface glycoprotein CD4	Inflammation in Aging
Q9Y6Q6	TNFRSF11A	Tumor necrosis factor receptor superfamily member 11A	Inflammation in Aging
O95630	STAMBP	STAM-binding protein	Inflammation in Aging
Q9H5V8	CDCP1	CUB domain-containing protein 1	Inflammation in Aging
P13500	CCL2	C-C motif chemokine 2	Inflammation in Aging
Q9NSA1	FGF21	Fibroblast growth factor 21	Inflammation in Aging
P14210	HGF	Hepatocyte growth factor	Inflammation in Aging
P05231	IL6	Interleukin-6	Inflammation in Aging
P01375	TNF	Tumor necrosis factor	Inflammation in Aging
P01579	IFNG	Interferon gamma	Inflammation in Aging
P01137	TGFB1	Transforming growth factor beta-1 proprotein	Inflammation in Aging
P22301	IL10	Interleukin-10	Inflammation in Aging
P09038	FGF2	Fibroblast growth factor 2	Inflammation in Aging
Q16552	IL17A	Interleukin-17A	Inflammation in Aging
P18510	IL1RA	Interleukin-1 receptor antagonist protein	Inflammation in Aging
P13232	IL7	Interleukin-7	Inflammation in Aging
P40933	IL15	Interleukin-15	Inflammation in Aging
P15018	LIF	Leukemia inhibitory factor	Inflammation in Aging
Q03405	PLAUR	Urokinase plasminogen activator surface receptor	Inflammation in Aging

Protein Functions

Biological process

Primarily involved in fundamental biological processes including cellular proliferation and differentiation, angiogenesis and vascular remodeling, immune cell activation and regulation, inflammatory response modulation, tissue repair and fibrosis, hematopoietic cell development, and cellular signaling transduction.

Disease area

Particularly relevant for scientific investigation across multiple research areas including autoimmune phenomena, cancer biology, metabolic disorders, cardiovascular pathologies, bone remodeling studies, neurodegenerative processes, and infectious disease responses.

The Application of Olink Flex Customized Inflammation in aging Panel.

The Olink Flex Customized Inflammation in Aging Panel enables comprehensive profiling of inflammaging-related biomarkers, providing researchers with a powerful tool for:

Systematic investigation of senescence-associated secretory phenotype (SASP) factors in age-related chronic inflammation;
Characterization of dysregulated immune pathways linking inflammaging to tissue degeneration;
Identification of molecular signatures associated with accelerated aging phenotypes.

Workflow of Olink Proteomics

Why Creativ Proteomics

Aging-Specific Analytical Frameworks

Preconfigured workflows for inflammaging research including SASP analysis, immunosenescence tracking, and oxidative stress responses with validated biomarker ratios.

DStandardized Inflammaging Metrics

Incorporates 21 published composite scores for biological age prediction and senescence burden assessment.

Validated Biomarker Ratios

Provides 68 pre-verified biomarker ratios relevant to chronic low-grade inflammation (inflammaging), reducing analytical validation burdens.

Cross-Platform Compatibility

Data outputs standardized for integration with major aging research databases (e.g., MouseAGE, Human Aging Genomic Resources).

Demo Results of Olink Data

Heatmap showing correlations between immune proteins and dietary inflammation scores in metabolic research. Figure 1: Association of inflammatory/immune proteins and pathways with energy-regulated dietary inflammatory index (EDII). (Duggan, M. R., et al. 2023)

Volcano plot showing significantly dysregulated proteins (NPX values) between l-GCF and h-GCF groups. Figure 2: Differential protein expression (NPX) visualized by volcano plot: l-GCF versus h-GCF. (Llaurador-Coll, M., et al. 2023)

Sample Requirements

Sample Type	Recommended Sample Size	Sample Quality	Pre-treatment and Storage	Sample Transport
Plasma/Serum/Body Fluid	Minimum volume: 40 µL/sample	Concentration range: 0.5–1 mg/mL	Portion sterile; maintain -80°C	Foil-sealed samples require dry ice (-80°C)
Tissue
Cells
Exosomes
Other

FAQs

What are the components of Olink Flex Negative controls and how are they used in data analysis?

Negative controls were contained in triplicate on each well plate and consisted of buffer runs of normal samples. These were used to monitor any background noise generated when the DNA tag was approached without prior binding to the appropriate protein. The negative control set the background level for each protein detection and was used to calculate the limit of detection (LOD).

What instruments are required to run Olink Flex?

If you are running our panel in your laboratory, Olink Signature Q100 needs to be used for detection and quantification. In the extension step of the test protocol, a validated PCR instrument with a 100µL reaction volume and a heated lid was also required.

BioMark HD Reader of Standard BioTool and IFC Controller HX/MX/AX/Juno can be used instead of Olink Signature Q100.

Can I customize the panel?

Yes! The panel allows: Selection of 15–21 markers from 208+ validated immune-related proteins. Adaptive configuration for specific research needs

References

Duggan, M. R., Butler, L., Peng, Z., et al. (2023). Plasma proteins related to inflammatory diet predict future cognitive impairment. Molecular psychiatry, 28(4), 1599–1609. https://doi.org/10.1038/s41380-023-01975-7
Llaurador-Coll, M., Rios, S., García-Gavilán, J. F., et al. (2023). Plasma levels of neurology-related proteins are associated with cognitive performance in an older population with overweight/obesity and metabolic syndrome. GeroScience, 45(4), 2457–2470. https://doi.org/10.1007/s11357-023-00764-y

* For research purposes only, not intended for clinical diagnosis, treatment, or individual health assessments.

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