Olink Target 48 Mouse Panel for Cytokine Assay

What is the Target 48 Mouse Cytokine Panel

Customized panel for mouse

The Olink Target 48 Mouse Cytokine Assay Panel empowers researchers to gain a comprehensive understanding of the mouse immune system, facilitating advanced disease modeling, longitudinal studies, and translational research. This innovative panel leverages the exceptional specificity and sensitivity of PEA technology, requiring just 1µL of sample to simultaneously measure 43 carefully selected mouse protein biomarkers that represent key biological processes and pathways.

Features of the panel

• Species: Specifically designed for mouse.
• Proteins: Simultaneously quantifies 43 Validated mouse-derived cytokines and immune-related proteins.
• Sample: Requires only 1µL of plasma, serum & more.
• Readout: Provide absolute concentrations (pg/mL) or relative units using Olink's proprietary Normalized Protein Expression (NPX) system.
• Platform: The panel is designed to run on the Olink Signature Q100 system.

List of 43 mouse derived biomarkers

Protein category

The Olink Target 48 Mouse Cytokine Panel includes 43 proteins categorized into four main groups: the interleukin family (19), chemokines (5), cytokines (10), and others (8), encompassing the immunoglobulin receptor superfamily, interferon family, growth factors, and intercellular signaling molecules. This extensive coverage of mouse immune system proteins makes the panel an essential tool for advanced disease modeling, longitudinal studies, and translational research.

Table. List of Olink Target 48 Mouse Cytokine Panel

Protein Functions

Biological process

Primarily associated with chemotaxis, immune cell activation, cytokine-mediated signaling pathways, innate immune responses, and pathways related to Th1, Th2, Th17, and Treg cells.

Disease area

Primarily associated with autoimmune diseases, immuno-oncology, antiviral responses, neuroinflammation, metabolic regulation, and tissue repair.

Kegg pathway

Widely distributed across various pathways, such as the Cytokine-cytokine receptor interaction pathway, JAK-STAT signaling pathway and son on.

Workflow of Olink Proteomics

Demo Results of Olink Data

Cytokine absolute concentration(Seldeslachts L, et al.2024)

Case Study

Dermal White Adipose Tissue–Derived Il-33 Regulates Il-4/13 Expression in Myeloid Cells during Inflammation

Journal: The Journal of investigative dermatology
Year: 2024

• Background
• Results

Adipocytes in white adipose tissue traditionally store energy as lipids but are now recognized as a dynamic endocrine organ, secreting adipokines that regulate nutrient homeostasis, energy storage, and inflammation. Dermal white adipose tissue (dWAT), a distinct fat depot, plays a crucial role in skin homeostasis, thermoregulation, tissue repair, and immune defense. Studies show that dWAT expands in response to injury or inflammation, supporting wound healing and antibacterial defense. Despite extensive research on subcutaneous and visceral fat, the immune-regulatory function of dWAT remains underexplored, prompting investigations into its role in myeloid cell activation and its interaction with inflammation, particularly under obese conditions.

The study's key findings highlight the activation of dermal white adipose tissue (dWAT) in response to skin inflammation. In a mouse model of acute skin inflammation induced by imiquimod (IMQ), significant changes were observed in dWAT, including myeloid cell infiltration and increased levels of proinflammatory mediators like S100A9. RNA sequencing revealed that 1291 genes were upregulated and 1036 genes were downregulated in dWAT, with the activation of critical pathways such as TLR, chemokine, and Fcg receptor signaling. Analysis of the secretome using the Olink Target 48 Mouse Cytokine Panel identified elevated levels of proinflammatory cytokines and chemokines, including IL-1b, TNFa, IL-1a, IFNγ, IL-12, and CCL4 in dWAT from lesional skin, confirming the strong proinflammatory activation of dWAT during skin inflammation.

Figure 1. Secretome analysis of dWAT from control and IMQ-treated mice after 24-hour culture using the Olink platform.(Ertel, A, et al.2024)

FAQs

Why Creative Proteomics

Comprehensive Bioinformatics Support

Backed by an experienced bioinformatics team, Creative Proteomics provides in-depth analysis of Olink data, enabling clients to gain a thorough understanding of complex immune mechanisms and key biological processes.

Broad Applications in Scientific Research

The services are ideal for disease modeling, longitudinal studies, and translational research, covering basic research, preclinical studies, and other scientific exploration needs.

Efficient Workflow and Standardized Services

Leveraging the advanced Olink Signature Q100 platform, Creative Proteomics ensures rapid, efficient sample testing with accurate and reliable results.

Industry-Leading Customer Support

Offering end-to-end support from sample preparation to data analysis, the company ensures comprehensive technical assistance and resource sharing throughout the research process.

Sample Requirements

Reference

1. Seldeslachts L, Staels F, Gkountzinopoulou M, et al. Damping excessive viral-induced IFN-γ rescues the impaired anti-Aspergillus host immune response in influenza-associated pulmonary aspergillosis. EBioMedicine. 2024;108:105347. https://doi.org/10.1016/j.ebiom.2024.105347
2. Ertel, A., Anderegg, U., Franz, S., & Saalbach, A. (2024). Dermal White Adipose Tissue-Derived Il-33 Regulates Il-4/13 Expression in Myeloid Cells during Inflammation. The Journal of investigative dermatology, S0022-202X(24)01862-1. Advance online publication. https://doi.org/10.1016/j.jid.2024.05.026