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What is the Olink Flex Customized Inflammation Panel
Customized panel for human
The Olink Flex Customized Inflammation Panel employs advanced multiplex proteomics to analyze 26 verified proteins - both established and novel biomarkers - in merely 1μl of plasma/serum, processing 40 samples per run. The platform demonstrates exceptional performance with <1 pg/mL detection limits and <5% CV reproducibility through normalized protein quantification. Featuring modular configuration, it enables researchers to create tailored panels by selecting 15-21 analytes from our extensive 208-marker inflammation collection, facilitating detailed mechanistic studies of inflammatory processes.
Features of the pane
- Species: Optimized for human studies.
- Design: Customizable via Olink Insight digital interface.
- Proteins: 26 pre-validated protein biomarkers for immediate research use.
- Sample: 1 µL plasma/serum required.
- Readout: NPX-normalized quantification.
- Platform: Olink Signature Q100 platform exclusive.
List of 26 Human Derived Biomarkers
Protein category
The Olink Flex Customized Inflammation Panel analyzes 26 strategically selected protein biomarkers categorized into key functional groups: Pro-inflammatory Cytokines/Chemokines, Anti-inflammatory Mediators, Acute-Phase Proteins, Complement System Components, Growth Factors, Adhesion Molecules, Pattern Recognition Receptors, and Stress Response Proteins (see Table. List of Olink Flex Customized Inflammation Panel).
These targets encompass human proteins involved in fundamental inflammatory pathways, including leukocyte activation, cytokine signaling, tissue remodeling, cellular stress responses, and immune regulation. The panel incorporates rigorously validated biomarkers associated with innate immunity, neutrophil activation, macrophage polarization, and endothelial dysfunction through systematic literature review and proteomic validation, offering researchers a comprehensive solution for investigating inflammatory mechanisms across diverse biological systems and disease models. This carefully curated selection enables multidimensional profiling of inflammatory networks while maintaining flexibility for customized panel configurations tailored to specific research objectives in immunology and inflammation biology.
Table. List of Olink Flex Customized Inflammation Panel
| UniProt ID | Gene | Protein name | Customized Olink Flex panel |
| P10147 | CCL3 | C-C motif chemokine 3 | Inflammation |
| P51671 | CCL11 | Eotaxin | Inflammation |
| P05231 | IL6 | Interleukin-6 | Inflammation |
| P10145 | CXCL8 | Interleukin-8 | Inflammation |
| P01375 | TNF | Tumor necrosis factor | Inflammation |
| P13236 | CCL4 | C-C motif chemokine 4 | Inflammation |
| P01584 | IL1B | Interleukin-1 beta | Inflammation |
| P01583 | IL1A | Interleukin-1 alpha | Inflammation |
| P13500 | CCL2 | C-C motif chemokine 2 | Inflammation |
| P22301 | IL10 | Interleukin-10 | Inflammation |
| P40933 | IL15 | Interleukin-15 | Inflammation |
| Q16552 | IL17A | Interleukin-17A | Inflammation |
| P01579 | IFNG | Interferon gamma | Inflammation |
| P35225 | IL13 | Interleukin-13 | Inflammation |
| P60568 | IL2 | Interleukin-2 | Inflammation |
| P13232 | IL7 | Interleukin-7 | Inflammation |
| Q99616 | CCL13 | C-C motif chemokine 13 | Inflammation |
| Q14005 | IL16 | Pro-interleukin-16 | Inflammation |
| P15692 | VEGFA | Vascular endothelial growth factor A | Inflammation |
| P05112 | (IL4) | Interleukin-4 | Inflammation |
| P05113 | IL5 | Interleukin-5 | Inflammation |
| Q92583 | CCL17 | C-C motif chemokine 17 | Inflammation |
| P04141 | CSF2 | Granulocyte-macrophage colony-stimulating factor | Inflammation |
| P01374 | LTA | Lymphotoxin-alpha | Inflammation |
| Q9Y258 | CCL26 | C-C motif chemokine 26 | Inflammation |
| P29460 | IL12 | Interleukin-12 | Inflammation |
Protein Functions
Biological process
primarily involved in fundamental biological processes including immune cell recruitment and chemotaxis, pro- and anti-inflammatory signaling cascades, lymphocyte activation and differentiation, Th1/Th2/Th17 immune responses, cytokine-mediated signaling pathways, angiogenesis regulation, hematopoietic cell development, acute phase response modulation, and cellular stress responses.
Disease area
particularly relevant for scientific investigations across multiple disease research areas including autoimmune conditions, allergic responses, chronic inflammatory disorders, infectious disease pathogenesis, tissue injury and repair mechanisms, metabolic inflammation, neurological inflammation processes, and vascular inflammation pathways.
The Application of Olink Flex Customized Inflammation Panel.
The Olink Flex Customized Inflammation Panel facilitates comprehensive profiling of inflammatory protein biomarkers, providing researchers with a versatile tool for:
- Systematic investigation of cytokine networks in autoimmune disease mechanisms;
- Characterization of inflammatory pathways involved in tissue injury and repair processes;
- Identification of molecular signatures associated with chronic inflammatory conditions.
Workflow of Olink Proteomics
Why Creativ Proteomics
Disease-Optimized Workflows
Preconfigured analytical frameworks for autoimmune (RA, IBD), infectious (sepsis, COVID-19), and chronic inflammation research, accelerating study setup.
Comprehensive Biomarker Coverage
Includes 68 validated biomarker ratios, enabling deeper mechanistic insights into inflammatory pathways.
Visualization Toolkits
Provides intuitive tools for data exploration and graphical representation, streamlining publication-ready outputs.
Broad Biomarker Ratios Validation
The inclusion of pre-validated biomarker ratios reduces the need for additional verification steps, saving time in exploratory research.
Demo Results of Olink Data
Figure 1: Protein heat maps of all tests in Ebola virus disease survivors, stratified by treatment, close contacts, and healthy controls. (Mwanza, J. C, et al. 2025)
Figure 2: The volcano plot depicts the differential expression of inflammatory proteins in EVD survivors versus controls. (Mwanza, J. C, et al. 2025)
Sample Requirements
| Sample Type | Recommended Sample Size | Sample Quality | Pre-treatment and Storage | Sample Transport |
| Plasma/Serum/Body Fluid | 40 µL minimum volume needed | 0.5 to 1 mg/mL protein content | Divide into sterile containers; maintain at -80°C | Transport at -80°C using dry ice (foil-sealed) |
| Tissue | ||||
| Cells | ||||
| Exosomes | ||||
| Other |
FAQs
Is there a software program to pre-process and analyze data from the Olink Flex?
To facilitate data analysis, Olink Proteomics has developed NPX signature software that can be used for data generated with Olink Flex products. This is an easy-to-use tool that allows you to import, quarantine, and normalize your Olink data, providing you with normalized protein expression (NPX) or pg/mL values (depending on your choice of Olink product) that can be used for further statistical analysis in the same software.
Is it possible to run formalin-fixed samples with Olink?
For sample analysis with Olink PEA technology (Target 96/Target 48/Explore/Focus/Flex), we have not yet found a satisfactory way to remove formalin while maintaining the structural integrity of the protein.
How should data below or above the lower limit be used for downstream analysis?
Overall recommendation is not to extrapolate the data, but to use a statistical method, such as a mixed-effects model, which can deal with missing data points. If you do want to estimate, we recommend comparing the results of estimated and non-estimated data. In this case, 0 can be calculated for data below the LLOQ and ULOQ for data above the ULOQ.
Reference
- Mwanza, J. C., Kahindo, A. K., Mbusa-Kombi, J., et al. (2025). Ophthalmological manifestations and plasma markers of inflammation in Ebola survivors in post-treatment era. Scientific reports, 15(1), 14966. https://doi.org/10.1038/s41598-025-96256-4