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What is the Olink Flex Customized Pro-inflammatory response Panel
Customized panel for human
The Olink Flex Customized Pro-inflammatory response Panel utilizes multiplexed immunoassays to quantify five validated biomarkers - including five validated biomarkers - in just 1μL plasma/serum, processing 40 samples per run. The high-sensitivity platform achieves <1 pg/mL detection limits and <5% CV reproducibility through normalized protein expression. Researchers can configure tailored panels by selecting 15-21 analytes from our 208-protein inflammation library, enabling focused investigations of pro-inflammatory mechanisms. This advanced solution combines established markers with novel targets for comprehensive pathway analysis, maintaining rigorous validation standards throughout. The system's precision and customization capabilities support detailed study of inflammatory responses across diverse research applications.
Features of the pane
- Species: Human-specific studies.
- Design: Design panels using Olink Insight.
- Proteins: the 5 biomarkers relevant to immune pathway analysis.
- Sample: 1 μL biological fluid (plasma/serum).
- Readout: NPX analytical method.
- Platform: Olink Signature Q100 platform exclusive.
List of 5 Human Derived Biomarkers
Protein category
The Olink Flex Customized Pro-inflammatory response Panel quantifies 5 strategically selected protein biomarkers organized into key functional categories: Pro-inflammatory Cytokines, Chemokine Ligands/Receptors, Acute-Phase Reactants, Complement System Proteins, Adhesion Molecules, Pattern Recognition Receptors, Inflammatory Mediators, and Stress Response Proteins (see Table. List of Olink Flex Customized Pro-inflammatory response Panel). These targets encompass human proteins involved in critical inflammatory pathways relevant to immunological research, covering processes such as leukocyte activation, cytokine signaling cascades, endothelial dysfunction, cellular stress responses, and tissue remodeling mechanisms.
The panel incorporates rigorously validated biomarkers associated with neutrophil activation, macrophage polarization, inflammasome signaling, and systemic inflammatory responses through exhaustive literature curation and proteomic verification, offering researchers a robust solution for comprehensive investigations of pro-inflammatory mechanisms across various biological systems and disease models. This carefully designed panel enables multidimensional analysis of inflammatory networks while maintaining the flexibility to create customized configurations tailored to specific research applications in inflammation biology and immunology studies.
Table. List of Olink Flex Customized Pro-inflammatory response Panel
| UniProt ID | Gene | Protein name | Customized Olink Flex panel |
| P01579 | IFNG | Interferon Gamma | Pro-inflammatory response |
| P01375 | TNF | Tumor Necrosis Factor | Pro-inflammatory response |
| P01584 | IL1B | Interleukin-1 beta | Pro-inflammatory response |
| P05231 | IL6 | Interleukin-6 | Pro-inflammatory response |
| P10145 | IL8 | Interleukin-8 | Pro-inflammatory response |
Protein Functions
Biological process
Primarily involved in fundamental biological processes including innate immune system activation, acute phase response signaling, leukocyte recruitment and chemotaxis, fever response regulation, cellular stress responses, and tissue remodeling mechanisms.
Disease area
particularly valuable for scientific investigation across multiple research areas including autoimmune phenomena, chronic inflammatory conditions, infectious disease pathogenesis, tissue damage and repair mechanisms, and systemic inflammatory responses.
The Application of Olink Flex Customized Pro-inflammatory response Panel.
The Olink Flex Customized Pro-inflammatory response Panel facilitates comprehensive analysis of inflammatory pathways, supporting researchers in:
- Elucidating immune cell activation and signaling cascades in various biological systems;
- Monitoring dynamic changes in inflammatory mediator expression during experimental interventions;
- Validating potential biomarkers of inflammatory status in preclinical models.
Workflow of Olink Proteomics
Why Creative Proteomics
Disease-Specific Analytical Frameworks
Preconfigured workflows for autoimmune (RA, IBD), infectious (sepsis, COVID-19), and chronic inflammation studies, enabling rapid hypothesis testing.
Inter-Plate Normalization
Minimizes batch effects, ensuring cross-study comparability in large-scale or multi-center research projects.
Scalable for Omics Integration
Compatible with multi-omics approaches (e.g., transcriptomics, proteomics, metabolomics), facilitating systems-level inflammation research.
Broad Target Coverage
Covers key pro-inflammatory mediators (cytokines, chemokines, signaling proteins), supporting comprehensive pathway analysis.
Demo Results of Olink Data
The differential expression of circulating inflammatory proteins in type 1 diabetes versus healthy controls. (Verhulst, C. E. M, et al. 2023)
Sample Requirements
| Sample Type | Recommended Sample Size | Sample Quality | Pre-treatment and Storage | Sample Transport |
| Plasma/Serum/Body Fluid | Minimum 40 µL per specimen required | Protein concentration range: 0.5–1 mg/mL | Distribute into sterile vials and freeze at -80°C | Dry ice shipment required (-80°C) for foil-sealed samples |
| Tissue | ||||
| Cells | ||||
| Exosomes | ||||
| Other |
FAQs
What tools do I need to run Olink Flex?
Detection and quantitation require the use of Olink signature Q100. In the expansion step of the protocol, a validated PCR instrument is also required that can use a reaction volume of 100 μL and a heated lid.
For a complete list of equipment and consumables required for each Olink product, please contact our support team.
How are the biomarkers in the Olink Flex panel selected?
In composing the new panel, we gathered information from widely used public bioinformatics databases, including Uniprot, Human Protein Atlas, Gene Ontology (GO), ChEMBL, and the test mining tool TenWise. We also include biomarkers requested by our customers. If the panel is established for a specific disease area or key biological process, we will work with key opinion leaders.
How do I perform quality control on Olink Flex data?
Quality control is done using Olink NPX signature software. It ensures reliable data by using three internal and external controls in each operation.
- Operational Evaluation: The standard deviations of Incubation Control 1, Incubation Control 2, and Assay Control must be less than 0.2 NPX.
- Sample evaluation: The results of incubation control 2 and assay control should deviate from the median plate by no more than ±0.3 NPX. If the deviation exceeds this value, the sample is marked. If more than 1/6 of the sample is "labeled", the data may be unreliable, need to be analyzed and possibly re-run.
Reference
- Verhulst, C. E. M., van Heck, J. I. P., Fabricius, T. W., et al. (2023). Hypoglycaemia induces a sustained pro-inflammatory response in people with type 1 diabetes and healthy controls. Diabetes, obesity & metabolism, 25(11), 3114–3124. https://doi.org/10.1111/dom.15205